https://wiki.chemika.be/api.php?action=feedcontributions&feedformat=atom&user=Ben 2026-07-28T11:54:59Z Gebruikersbijdragen MediaWiki 1.43.0 https://wiki.chemika.be/index.php?title=Biocatalysis:_Science_and_Technology&diff=1540 2019-01-26T11:37:57Z

<p>Ben: /* 24/01/2019 */</p> <hr /> <div>[[Categorie: Mabb]]<br /> <br /> == Vakinformatie ==<br /> Advanced Enzymology<br /> <br /> ECTS-fiche: https://onderwijsaanbod.kuleuven.be/syllabi/e/G0U19AE.htm<br /> <br /> == Examenvragen ==<br /> <br /> ==24/01/2019==<br /> PART 1. Oral examination by prof. Peter Dedecker<br /> # Given a kinetic scheme with product feedback, compose the differential equations that govern this system.<br /> # Explain the value of k' = k1 (1+([S2]/K)^n) [S1].<br /> # Explain the concept and the procedure of numerical integration for a simulation of a product feedback scheme.<br /> # Given data of real, in vitro experiment of [Ca2+]i by Fura-2 and [cAMP] by IUCP, list the problems that may arise in such an experiment and how to surmount them. How does an experiment like this work practically?<br /> <br /> PART 2. Written examination by Dr. Fabian Hertel<br /> # Sketch an acid-base, electrophilic or nucleophilic catalysis pathway using a correct amino acid of choice.<br /> # Give the correct action mechanism for two different amino acids.<br /> # What role do metal ions play in enzymatic catalysis?<br /> # Sketch a 2D folding funnel. Mark where the completely folded enzyme, and a unfolded intermediate are located. What mechanisms exist in nature to make the folding process more efficient?<br /> # Your research requires an enzyme that is capable of converting MDMA to the non-pyschoactive compound benzaldehyde. There exists a catalytic antibody that is capable of this reaction. How do these catalytic Abs work? Is further optimisation necessary? Describe one method to design an artificial enzyme.</div>

Ben https://wiki.chemika.be/index.php?title=Biocatalysis:_Science_and_Technology&diff=1539 2019-01-26T11:37:17Z

<p>Ben: /* Examenvragen */</p> <hr /> <div>[[Categorie: Mabb]]<br /> <br /> == Vakinformatie ==<br /> Advanced Enzymology<br /> <br /> ECTS-fiche: https://onderwijsaanbod.kuleuven.be/syllabi/e/G0U19AE.htm<br /> <br /> == Examenvragen ==<br /> <br /> ==24/01/2019==<br /> #PART 1. Oral examination by prof. Peter Dedecker<br /> ## Given a kinetic scheme with product feedback, compose the differential equations that govern this system.<br /> ## Explain the value of k' = k1 (1+([S2]/K)^n) [S1].<br /> ## Explain the concept and the procedure of numerical integration for a simulation of a product feedback scheme.<br /> ## Given data of real, in vitro experiment of [Ca2+]i by Fura-2 and [cAMP] by IUCP, list the problems that may arise in such an experiment and how to surmount them. How does an experiment like this work practically?<br /> <br /> #PART 2. Written examination by Dr. Fabian Hertel<br /> ## Sketch an acid-base, electrophilic or nucleophilic catalysis pathway using a correct amino acid of choice.<br /> ## Give the correct action mechanism for two different amino acids.<br /> ## What role do metal ions play in enzymatic catalysis?<br /> ## Sketch a 2D folding funnel. Mark where the completely folded enzyme, and a unfolded intermediate are located. What mechanisms exist in nature to make the folding process more efficient?<br /> ## Your research requires an enzyme that is capable of converting MDMA to the non-pyschoactive compound benzaldehyde. There exists a catalytic antibody that is capable of this reaction. How do these catalytic Abs work? Is further optimisation necessary? Describe one method to design an artificial enzyme.</div>

Ben