https://wiki.chemika.be/api.php?action=feedcontributions&feedformat=atom&user=R0721237 2026-07-28T12:49:04Z Gebruikersbijdragen MediaWiki 1.43.0 https://wiki.chemika.be/index.php?title=Biocatalysis:_Science_and_Technology&diff=2812 2022-01-27T15:08:37Z

<p>R0721237: /* 27/01/2022 */</p> <hr /> <div>[[Categorie: Mabb]]<br /> <br /> == Vakinformatie ==<br /> Voor 2021 heette het vak Advanced Enzymology.<br /> <br /> ECTS-fiche: https://onderwijsaanbod.kuleuven.be/syllabi/e/G0U19AE.htm<br /> <br /> == Examenvragen ==<br /> ===27/01/2022===<br /> '''Question 1'''<br /> <br /> # What drives protein folding? Which mechanisms are involved? <br /> # Why do we say that the structure of a protein is encoded in the primary sequence?<br /> # How do AlphaFold and related algorithms work? (not a detailed description, just generally)<br /> <br /> '''Question 2'''<br /> <br /> Why do proteins need cofactors? Explain with examples.<br /> <br /> '''Question 3'''<br /> <br /> Figure of Ohno and IAD model is given.<br /> # What does neofunctionalization mean in the scheme below?<br /> # What does specialization mean?<br /> # Why does the IAD model, which is thought to be more correct than the Ohno model, indicate that neofunctionalization happens first?<br /> # What does this mean for the specificity of natural enzymes?<br /> <br /> ===24/01/2019===<br /> PART 1. Oral examination by prof. Peter Dedecker<br /> # Given a kinetic scheme with product feedback, compose the differential equations that govern this system.<br /> # Explain the value of k' = k1 (1+([S2]/K)^n) [S1].<br /> # Explain the concept and the procedure of numerical integration for a simulation of a product feedback scheme.<br /> # Given data of real, in vitro experiment of [Ca2+]i by Fura-2 and [cAMP] by IUCP, list the problems that may arise in such an experiment and how to surmount them. How does an experiment like this work practically?<br /> <br /> PART 2. Written examination by Dr. Fabian Hertel<br /> # Sketch an acid-base, electrophilic or nucleophilic catalysis pathway using a correct amino acid of choice.<br /> # Give the correct action mechanism for two different amino acids.<br /> # What role do metal ions play in enzymatic catalysis?<br /> # Sketch a 2D folding funnel. Mark where the completely folded enzyme, and a unfolded intermediate are located. What mechanisms exist in nature to make the folding process more efficient?<br /> # Your research requires an enzyme that is capable of converting MDMA to the non-pyschoactive compound benzaldehyde. There exists a catalytic antibody that is capable of this reaction. How do these catalytic Abs work? Is further optimisation necessary? Describe one method to design an artificial enzyme.</div>

R0721237 https://wiki.chemika.be/index.php?title=Biocatalysis:_Science_and_Technology&diff=2811 2022-01-27T15:07:46Z

<p>R0721237: /* Examenvragen */</p> <hr /> <div>[[Categorie: Mabb]]<br /> <br /> == Vakinformatie ==<br /> Voor 2021 heette het vak Advanced Enzymology.<br /> <br /> ECTS-fiche: https://onderwijsaanbod.kuleuven.be/syllabi/e/G0U19AE.htm<br /> <br /> == Examenvragen ==<br /> ===27/01/2022===<br /> '''Question 1'''<br /> <br /> # What drives protein folding? Which mechanisms are involved? <br /> # Why do we say that the structure of a protein is encoded in the primary sequence?<br /> # How do AlphaFold and related algorithms work?<br /> <br /> '''Question 2'''<br /> <br /> Why do proteins need cofactors? Explain with examples.<br /> <br /> '''Question 3'''<br /> <br /> Figure of Ohno and IAD model is given.<br /> # What does neofunctionalization mean in the scheme below?<br /> # What does specialization mean?<br /> # Why does the IAD model, which is thought to be more correct than the Ohno model, indicate that neofunctionalization happens first?<br /> # What does this mean for the specificity of natural enzymes?<br /> <br /> ===24/01/2019===<br /> PART 1. Oral examination by prof. Peter Dedecker<br /> # Given a kinetic scheme with product feedback, compose the differential equations that govern this system.<br /> # Explain the value of k' = k1 (1+([S2]/K)^n) [S1].<br /> # Explain the concept and the procedure of numerical integration for a simulation of a product feedback scheme.<br /> # Given data of real, in vitro experiment of [Ca2+]i by Fura-2 and [cAMP] by IUCP, list the problems that may arise in such an experiment and how to surmount them. How does an experiment like this work practically?<br /> <br /> PART 2. Written examination by Dr. Fabian Hertel<br /> # Sketch an acid-base, electrophilic or nucleophilic catalysis pathway using a correct amino acid of choice.<br /> # Give the correct action mechanism for two different amino acids.<br /> # What role do metal ions play in enzymatic catalysis?<br /> # Sketch a 2D folding funnel. Mark where the completely folded enzyme, and a unfolded intermediate are located. What mechanisms exist in nature to make the folding process more efficient?<br /> # Your research requires an enzyme that is capable of converting MDMA to the non-pyschoactive compound benzaldehyde. There exists a catalytic antibody that is capable of this reaction. How do these catalytic Abs work? Is further optimisation necessary? Describe one method to design an artificial enzyme.</div>

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