Advanced NMR Spectroscopy - Bewerkingsoverzicht

R0882878: /* Course information */

2026-01-25T20:10:35Z

Course information

← Oudere versie		Versie van 25 jan 2026 22:10
Regel 1:		Regel 1:
	[[Categorie:Machemie]]		[[Categorie:Machemie]]
	=Course information=		=Course information=
	In 2025-2026, the course Advanced NMR Spectroscopy consists of three parts: Part 1 General NMR theory (Prof. Wim De Borggraeve); Part 2 Applications of NMR (with guest lectures from Prof. Tatjana Parac-Vogt and Prof. Dimitrios Sakellariou and Dr. Gert Steurs); Part 3 Exercises (Dr. Gert Steurs). There is also a reading assignment from Prof. Vogt (scientific article about an application of NMR), with exam questions about the article.		In 2025-2026, the course Advanced NMR Spectroscopy consists of three parts: Part 1 General NMR theory (Prof. Wim De Borggraeve); Part 2 Applications of NMR (with guest lectures from Prof. Tatjana Parac-Vogt and Prof. Dimitrios Sakellariou and Dr. Gert Steurs); Part 3 Exercises (Dr. Gert Steurs). There is also a reading assignment from Prof. Vogt (scientific article about an application of NMR), with exam questions about the article. The exam takes 3 hours and this will be barely enough, so do not waste too much time on hard questions.

	==Exam questions==		==Exam questions==

R0882878: /* Course information */

2026-01-10T14:03:32Z

Course information

← Oudere versie		Versie van 10 jan 2026 16:03
Regel 1:		Regel 1:
	[[Categorie:Machemie]]		[[Categorie:Machemie]]
	=Course information=		=Course information=
	In 2025-2026, the course Advanced NMR Spectroscopy consists of three parts: Part 1 General NMR theory (Prof. Wim De Borggraeve); Part 2 Applications of NMR (with guest lectures from Prof. Tatjana Parac-Vogt and Prof. Dimitrios Sakellariou and Dr. Gert Steurs); Part 3 Exercises (Dr. Gert Steurs). There is also a reading assignment from Prof. Vogt (scientific article about an application of NMR).		In 2025-2026, the course Advanced NMR Spectroscopy consists of three parts: Part 1 General NMR theory (Prof. Wim De Borggraeve); Part 2 Applications of NMR (with guest lectures from Prof. Tatjana Parac-Vogt and Prof. Dimitrios Sakellariou and Dr. Gert Steurs); Part 3 Exercises (Dr. Gert Steurs). There is also a reading assignment from Prof. Vogt (scientific article about an application of NMR), with exam questions about the article.

	==Exam questions==		==Exam questions==

R0882878: /* Course information */

2026-01-10T14:02:39Z

Course information

← Oudere versie		Versie van 10 jan 2026 16:02
Regel 1:		Regel 1:
	[[Categorie:Machemie]]		[[Categorie:Machemie]]
	=Course information=		=Course information=
	In 2025-2026, the course Advanced NMR Spectroscopy consists of three parts: Part 1 General NMR theory (Prof. Wim De Borggraeve; Part 2 Applications of NMR (with guest lectures from Prof. Tatjana Parac-Vogt and Prof. Dimitrios Sakellariou and Dr. Gert Steurs); Part 3 Exercises (Dr. Gert Steurs). There is also a reading assignment from Prof. Vogt (scientific article about an application of NMR).		In 2025-2026, the course Advanced NMR Spectroscopy consists of three parts: Part 1 General NMR theory (Prof. Wim De Borggraeve); Part 2 Applications of NMR (with guest lectures from Prof. Tatjana Parac-Vogt and Prof. Dimitrios Sakellariou and Dr. Gert Steurs); Part 3 Exercises (Dr. Gert Steurs). There is also a reading assignment from Prof. Vogt (scientific article about an application of NMR).

	==Exam questions==		==Exam questions==

R0882878: /* Vakinformatie */

2026-01-10T14:02:20Z

Vakinformatie

← Oudere versie		Versie van 10 jan 2026 16:02
Regel 1:		Regel 1:
	[[Categorie:Machemie]]		[[Categorie:Machemie]]
	=~~Vakinformatie~~=		=Course information=
	Advanced NMR Spectroscopy		In 2025-2026, the course Advanced NMR Spectroscopy consists of three parts: Part 1 General NMR theory (Prof. Wim De Borggraeve; Part 2 Applications of NMR (with guest lectures from Prof. Tatjana Parac-Vogt and Prof. Dimitrios Sakellariou and Dr. Gert Steurs); Part 3 Exercises (Dr. Gert Steurs). There is also a reading assignment from Prof. Vogt (scientific article about an application of NMR).
	==~~Examenvragen~~==
			==Exam questions==

	===13 januari 2025===		===13 januari 2025===

R0846615 op 14 jan 2025 om 11:51

2025-01-14T11:51:15Z

← Oudere versie		Versie van 14 jan 2025 13:51
Regel 4:		Regel 4:
	==Examenvragen==		==Examenvragen==

	===13 januari ~~2024~~===		===13 januari 2025===
	Vogt (ppt + paper + SI on pc)		Vogt (ppt + paper + SI on pc)
	*Paper: Monitoring the interaction of alpha-synuclein with calcium ions through exclusively heteronuclear NMR experiments.		*Paper: Monitoring the interaction of alpha-synuclein with calcium ions through exclusively heteronuclear NMR experiments.
Regel 25:		Regel 25:
	*How the spectrometer works: given 5 definitions, link 4 of them to: field-freq lock, shimming, matching&tuning, receiver gain		*How the spectrometer works: given 5 definitions, link 4 of them to: field-freq lock, shimming, matching&tuning, receiver gain
	*BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?		*BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?
	*BONUS QUESTION: Calculate d2 (in milliseconds) from the exercise of ~~Deborggraeve~~ using provided data sets (I think you had to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))		*BONUS QUESTION: Calculate d2 (in milliseconds) from the exercise of De Borggraeve using provided data sets (I think you had to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))

	===16 januari 2023===		===16 januari 2023===

R0900076: /* 13 januari 2024 */

2025-01-14T06:47:52Z

13 januari 2024

← Oudere versie		Versie van 14 jan 2025 08:47
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	#How many times did different values of t1 get recorded in (file number)? (not sure about this question)		#How many times did different values of t1 get recorded in (file number)? (not sure about this question)
	#How many times did the spectrometer run through the pulse sequence in (file number)?		#How many times did the spectrometer run through the pulse sequence in (file number)?
	#How the spectrometer works: given 5 definitions, link 4 of them to: field-freq lock, shimming, matching&tuning, receiver gain		*How the spectrometer works: given 5 definitions, link 4 of them to: field-freq lock, shimming, matching&tuning, receiver gain
	#BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?		*BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?
	#BONUS QUESTION: Calculate d2 (in milliseconds) from the exercise of Deborggraeve using provided data sets (I think you had to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))		*BONUS QUESTION: Calculate d2 (in milliseconds) from the exercise of Deborggraeve using provided data sets (I think you had to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))

	===16 januari 2023===		===16 januari 2023===

R0900076: /* 13 januari 2024 */

2025-01-14T06:46:57Z

13 januari 2024

← Oudere versie		Versie van 14 jan 2025 08:46
Regel 5:		Regel 5:

	===13 januari 2024===		===13 januari 2024===
	Vogt		Vogt (ppt + paper + SI on pc)
	*Paper: Monitoring the interaction of alpha-synuclein with calcium ions through exclusively heteronuclear NMR experiments.		*Paper: Monitoring the interaction of alpha-synuclein with calcium ions through exclusively heteronuclear NMR experiments.
	#Draw the structure of aspartate and indicate C', C(alpha), C(bèta) and C(gamma). Use the spectra from the paper to derive the chemical shifts of these carbons. Which experiments were used to study the interactions of the protein with Ca2+?		#Draw the structure of aspartate and indicate C', C(alpha), C(bèta) and C(gamma). Use the spectra from the paper to derive the chemical shifts of these carbons. Which experiments were used to study the interactions of the protein with Ca2+?
	#Explain the role of proline and tyrosine in the calcium-protein binding. Why are the signals of these amino acids the ones that are most changed by this interaction?		#Explain the role of proline and tyrosine in the calcium-protein binding. Why are the signals of these amino acids the ones that are most changed by this interaction?

			Sakellariou (ppt on pc)
			#What is the main reason for line-broadening in solid state NMR and which technique is used to avoid this issue? Can nuclei with I = 1 be determined with MQMAS?

	De Borggraeve		De Borggraeve
	C-H Heteronuclear pulse program is given with: d1, p1 (= 90° on H), d2, p2 (on C). The delay d1 is 5T1 so that all spins are in equilibrium at p1. The delay d2 is given by 1/J(IS). The experiment is carried out for CHCl3, with offsets of 0 Hz for H and C.		C-H Heteronuclear pulse program is given with: d1, p1 (= 90° on H), d2, p2 (on C). The delay d1 is 5T1 so that all spins are in equilibrium at p1. The delay d2 is given by 1/J(IS). The experiment is carried out for CHCl3, with offsets of 0 Hz for H and C.
	#With which experiment(s) can we determine d2?		#With which experiment(s) can we determine d2?
	#When p2<90° the spectrum contains one absorptive peak up and one absorptive peak down, when p2>90° these peaks get inverted. When p2=90°, the peaks disappear. Prove this with product operators.		#Assume now that we know d2. When p2 < 90° the spectrum contains one absorptive peak up and one absorptive peak down, when p2 > 90° these peaks get inverted. When p2 = 90°, the peaks disappear. Prove this with product operators.

	Steurs		Steurs

R0900076: /* 13 januari 2024 */

2025-01-14T06:36:02Z

13 januari 2024

← Oudere versie		Versie van 14 jan 2025 08:36
Regel 13:		Regel 13:
	C-H Heteronuclear pulse program is given with: d1, p1 (= 90° on H), d2, p2 (on C). The delay d1 is 5T1 so that all spins are in equilibrium at p1. The delay d2 is given by 1/J(IS). The experiment is carried out for CHCl3, with offsets of 0 Hz for H and C.		C-H Heteronuclear pulse program is given with: d1, p1 (= 90° on H), d2, p2 (on C). The delay d1 is 5T1 so that all spins are in equilibrium at p1. The delay d2 is given by 1/J(IS). The experiment is carried out for CHCl3, with offsets of 0 Hz for H and C.
	#With which experiment(s) can we determine d2?		#With which experiment(s) can we determine d2?
	#When p2<90° ~~there is an anti-phase~~ spectrum, when p2>90° ~~this anti-phase gets~~ inverted. When p2=90°, the peaks disappear. Prove this with product operators.		#When p2<90° the spectrum contains one absorptive peak up and one absorptive peak down, when p2>90° these peaks get inverted. When p2=90°, the peaks disappear. Prove this with product operators.

	Steurs		Steurs
Regel 20:		Regel 20:
	#How many times did different values of t1 get recorded in (file number)? (not sure about this question)		#How many times did different values of t1 get recorded in (file number)? (not sure about this question)
	#How many times did the spectrometer run through the pulse sequence in (file number)?		#How many times did the spectrometer run through the pulse sequence in (file number)?
			#How the spectrometer works: given 5 definitions, link 4 of them to: field-freq lock, shimming, matching&tuning, receiver gain
	#BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?		#BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?
	#BONUS QUESTION: Calculate d2 (in milliseconds) from the exercise of Deborggraeve using provided data sets (I think you had to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))		#BONUS QUESTION: Calculate d2 (in milliseconds) from the exercise of Deborggraeve using provided data sets (I think you had to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))

R0900076: /* 13 januari 2024 */

2025-01-13T18:49:30Z

13 januari 2024

← Oudere versie		Versie van 13 jan 2025 20:49
Regel 21:		Regel 21:
	#How many times did the spectrometer run through the pulse sequence in (file number)?		#How many times did the spectrometer run through the pulse sequence in (file number)?
	#BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?		#BONUS QUESTION: What is the spin system of F2C=C=CH2 knowing that the spin system of F2C=CH2 is AA'XX'?
	#BONUS QUESTION: Calculate d2 from the exercise of Deborggraeve using provided data sets (I think you ~~need~~ to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))		#BONUS QUESTION: Calculate d2 (in milliseconds) from the exercise of Deborggraeve using provided data sets (I think you had to measure J(C-H) from the C-NMR spectrum -> d2 = 1/2J(C-H))

	===16 januari 2023===		===16 januari 2023===

R0900076: /* 13 januari 2024 */

2025-01-13T18:44:46Z

13 januari 2024

← Oudere versie		Versie van 13 jan 2025 20:44
Regel 11:		Regel 11:

	De Borggraeve		De Borggraeve
	C-H Heteronuclear pulse program is given with: d1, p1 (= 90° on H), d2, p2 (on C). The delay d1 is 5T1 so that spins are in equilibrium at p1. The delay d2 is given by 1/J(IS). The experiment is carried out for CHCl3, with offsets of 0 Hz for H and C.		C-H Heteronuclear pulse program is given with: d1, p1 (= 90° on H), d2, p2 (on C). The delay d1 is 5T1 so that all spins are in equilibrium at p1. The delay d2 is given by 1/J(IS). The experiment is carried out for CHCl3, with offsets of 0 Hz for H and C.
	#With which experiment(s) can we determine d2?		#With which experiment(s) can we determine d2?
	#When p2<90° there is an anti-phase spectrum, when p2>90° this anti-phase gets inverted. When p2=90°, the peaks disappear. Prove this with product operators.		#When p2<90° there is an anti-phase spectrum, when p2>90° this anti-phase gets inverted. When p2=90°, the peaks disappear. Prove this with product operators.