Model Organisms in Biological Research: verschil tussen versies

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Regel 18: Regel 18:


- Knowing the results, what technique or method would you add to this research
- Knowing the results, what technique or method would you add to this research
- Give 2 arguments why this organism is a model organisms.


Theoretical question:
Theoretical question:


- Explain terminator seeds
- Explain terminator seeds
- After doing a forward screening in C. elegans, they found mutants with altered body length. Explain the strategy how you can know the causal mutations. Can you perform this stragety in other organisms and which step is important for causality?





Versie van 29 jan 2021 12:19


Vakinformatie

Model Organisms in biological research

ECTS-fiche: https://onderwijsaanbod.kuleuven.be/syllabi/e/G0G43AE.htm

Examenvragen

2021 January

Self chosen article:

- Why is this organism chosen for this research question

- Why is this organism chosen instead of another model organism

- Do you agree with the choice of the author

- Knowing the results, what technique or method would you add to this research

- Give 2 arguments why this organism is a model organisms.

Theoretical question:

- Explain terminator seeds

- After doing a forward screening in C. elegans, they found mutants with altered body length. Explain the strategy how you can know the causal mutations. Can you perform this stragety in other organisms and which step is important for causality?


2020 January

What is a polytene chromosome and how is it used for genetics? (Extra questions: Why was it easier to have this to study genetics, answer because it is much bigger thanks to endoduplication which means that you can see it under a microscope. If you would have found a piece of the chromosome and you can sequence it, how can you find where it is located on the polytene chromosome, answer was in situ hybridisation [You can't answer CRISPR or something like that because it didn't exist back then].)

ES technology, any alternatives?

Give three tetrads

- explain the Tet system

- explain tetrad analysis

- explain transformation in Arabidopsis thaliana.

- Explain P elements

- QTL mapping

2019 January

ES technology mice

QTL mapping

Optogenetics

give examples of prokaryotes in biological research and explain their use

what are terminator seeds, what is their use and given an example of how they work

2017-2018 January

Wich primates are used as model organism and for which applications?

Explain Tetrad analysis and the application.

Explain Pronucleus injection in mice and the application.

Explain foward genetics in C. elegans.

Explain Cre recombinase system and how to use it giving examples