Biocatalysis: Science and Technology: verschil tussen versies
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== Examenvragen == | == Examenvragen == | ||
==24/01/2019== | |||
#PART 1. Oral examination by prof. Peter Dedecker | |||
## Given a kinetic scheme with product feedback, compose the differential equations that govern this system. | |||
## Explain the value of k' = k1 (1+([S2]/K)^n) [S1]. | |||
## Explain the concept and the procedure of numerical integration for a simulation of a product feedback scheme. | |||
## Given data of real, in vitro experiment of [Ca2+]i by Fura-2 and [cAMP] by IUCP, list the problems that may arise in such an experiment and how to surmount them. How does an experiment like this work practically? | |||
#PART 2. Written examination by Dr. Fabian Hertel | |||
## Sketch an acid-base, electrophilic or nucleophilic catalysis pathway using a correct amino acid of choice. | |||
## Give the correct action mechanism for two different amino acids. | |||
## What role do metal ions play in enzymatic catalysis? | |||
## Sketch a 2D folding funnel. Mark where the completely folded enzyme, and a unfolded intermediate are located. What mechanisms exist in nature to make the folding process more efficient? | |||
## Your research requires an enzyme that is capable of converting MDMA to the non-pyschoactive compound benzaldehyde. There exists a catalytic antibody that is capable of this reaction. How do these catalytic Abs work? Is further optimisation necessary? Describe one method to design an artificial enzyme. | |||
Versie van 26 jan 2019 13:37
Vakinformatie
Advanced Enzymology
ECTS-fiche: https://onderwijsaanbod.kuleuven.be/syllabi/e/G0U19AE.htm
Examenvragen
24/01/2019
- PART 1. Oral examination by prof. Peter Dedecker
- Given a kinetic scheme with product feedback, compose the differential equations that govern this system.
- Explain the value of k' = k1 (1+([S2]/K)^n) [S1].
- Explain the concept and the procedure of numerical integration for a simulation of a product feedback scheme.
- Given data of real, in vitro experiment of [Ca2+]i by Fura-2 and [cAMP] by IUCP, list the problems that may arise in such an experiment and how to surmount them. How does an experiment like this work practically?
- PART 2. Written examination by Dr. Fabian Hertel
- Sketch an acid-base, electrophilic or nucleophilic catalysis pathway using a correct amino acid of choice.
- Give the correct action mechanism for two different amino acids.
- What role do metal ions play in enzymatic catalysis?
- Sketch a 2D folding funnel. Mark where the completely folded enzyme, and a unfolded intermediate are located. What mechanisms exist in nature to make the folding process more efficient?
- Your research requires an enzyme that is capable of converting MDMA to the non-pyschoactive compound benzaldehyde. There exists a catalytic antibody that is capable of this reaction. How do these catalytic Abs work? Is further optimisation necessary? Describe one method to design an artificial enzyme.