Applied Medical Biotechnology

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ECTS-fiche:

Examenvragen

12 januari 2024

Cools:

1. Ik heb 10 kankercellijnen waarvan er 4 een puntmutatie hebben op een non-coded region. De puntmutatie bevindt zich voor de promotor van een gekend oncogen. Hoe controleer ik of de puntmutatie invloed heeft op de expressie van dit oncogen? Beschrijf de methodes die je zou toepassen (ChIP sequencing + ATAC)

1. Definitions

a) oncohistones b) single-cell CRISPR screen c) bispecific antibodies d) PROTAC

Van den Steen:

1. Give two examples of therapeutics of type 1 interferon. Should IFN-bèta be glycosylated when used as therapeutic? Explain why (not)
2. What is the link between sequestration and evading the immune system regarding malaria? Could the molecules used for sequestration be a good vaccin target? Explain in detail why (not).

Lambrechts:

1. What is measured with CITEseq, ATACseq and scTCRseq? Explain briefly
2. How is zygote injection done to generate a transgenic mice? Give briefly 5 examples.

19 januari 2023

Cools:

1. a) PROTAC b) Problems when using in cancer treatment
2. ChIP-sequencing
3. ShRNA screen against 100 genes of which 1 causing drug resistance
4. a) What is clonal hematopoiesis? b) 100 people, how do you find out who has CH? Describe step by step: what do you need/what technology do you use/how do you perform data analysis?

Eelen:

1. What is meant by 'Gene transfer in somatic cells'? Give an example. - max. 5 lines
2. How can the Cre/LoxP system be made inducible? Can it be made tissue specific? - max. 5 lines
3. Explain the Tet-on/Tet-off system schematically. Can it be made tissue specific? - max. 1 page

Lambrechts:

1. PRS? How did it originate? How can it be used for breast cancer?
2. Methylation as chemical marker? Chemical reaction? Use?

Van Den Steen:

1. Passive immunisation therapy against cerebral malaria
2. Difluoro-methylornithine