Biocatalysis: Science and Technology

Vakinformatie

Advanced Enzymology

ECTS-fiche: https://onderwijsaanbod.kuleuven.be/syllabi/e/G0U19AE.htm

24/01/2019

1. PART 1. Oral examination by prof. Peter Dedecker
   1. Given a kinetic scheme with product feedback, compose the differential equations that govern this system.
   2. Explain the value of k' = k1 (1+([S2]/K)^n) [S1].
   3. Explain the concept and the procedure of numerical integration for a simulation of a product feedback scheme.
   4. Given data of real, in vitro experiment of [Ca2+]i by Fura-2 and [cAMP] by IUCP, list the problems that may arise in such an experiment and how to surmount them. How does an experiment like this work practically?

1. PART 2. Written examination by Dr. Fabian Hertel
   1. Sketch an acid-base, electrophilic or nucleophilic catalysis pathway using a correct amino acid of choice.
   2. Give the correct action mechanism for two different amino acids.
   3. What role do metal ions play in enzymatic catalysis?
   4. Sketch a 2D folding funnel. Mark where the completely folded enzyme, and a unfolded intermediate are located. What mechanisms exist in nature to make the folding process more efficient?
   5. Your research requires an enzyme that is capable of converting MDMA to the non-pyschoactive compound benzaldehyde. There exists a catalytic antibody that is capable of this reaction. How do these catalytic Abs work? Is further optimisation necessary? Describe one method to design an artificial enzyme.